Flash dietary methionine supply over growth requirements in pigs: Multi-facetted effects on skeletal muscle metabolism

Dietary methionine affects protein metabolism, lean gain and growth performance acts in the control of oxidative stress. When supplied large excess relative to requirements diets for pigs, positive effects on pork quality traits have been recently reported. This study aimed decipher molecular biochemical mechanisms affected by a dietary supply above loin muscle finishing pigs. During last 14 days before slaughter, crossbred female pigs (n = 15 pigs/diet) were fed diet supplemented with hydroxy-methionine (Met5; 1.1% methionine) or not (CONT, 0.22% methionine). Blood was sampled at slaughter assess key metabolites. At same time, free amino acid concentrations expression activity levels genes involved energy metabolism measured longissimus lumborum (LM). The Met5 exhibited greater creatine kinase plasma when compared CONT methionine, alpha-aminobutyric acid, anserine, 3-methyl-histidine, lysine, proline LM than Expression synthesis, breakdown autophagy only scarcely diet. Among ubiquitin ligases, MURF1, gene known target contractile proteins, OTUD1 coding deubiquitinase protease, up-regulated A lower citrate synthase, reduced level ME1 acting lipogenesis but higher PPARD regulating also observed Principal component analysis revealed that many studied correlated meat across treatments, suggesting subtle modifications those had cumulative regulation processes leading transformation into meat. In conclusion, supplementation beyond nutritional during modified profile its redox capacities, slightly pathways related metabolism. These associated benefits traits.